This Week in Virology

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler

The complete TWiV team talks about how two different viruses shape the evolution of an essential housekeeping protein.

• Dual virus-host arms race (PLoS Biology)
• Dual virus-receptor duel (see comments - virology blog)
• Ferritin and transferrin (Protein Data Bank)
• Money for nothing (YouTube)
• Letters read on TWiV 242

Dickson - Mantid imagesKathy - No email? (phd comics)Alan - The turbid plaqueVincent - AAM Faq: West Nile VirusRich - Science is awesome

Luis - Public Health Image LibraryStephen - Hopkins closes graduate science-writing program

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Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler

Vincent, Alan, Rich and Kathy review how human placental trophoblasts confer viral resistance via exosome-mediated delivery of microRNAs, and isolation of the first human influenza virus in 1933.

Links for this episode:

• Human placental trophoblasts confer viral resistance (PNAS)
• Placental circulation (Wikipedia)
• Ovum in uterine decidua (Wikipedia)
• A virus obtained from influenza patients (Lancet)
• 80 years ago today (the guardian)
• Influenza: Exposing the true killer (J Exp Med)
• Letters read on TWiV 239

Matt - The Kid Should See ThisAlan - Cassini to photograph Earth Vincent -Cockroaches! (YouTube)Rich - Sydney Brenner (Web of Stories)

Joe - Grand challenges in science education (Science)Mark - Weather Underground

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Host: Vincent Racaniello

Guests: Markus Thali and Jason Botten

Vincent travels to the University of Vermont to talk with Markus and Jason about their work on HIV, influenza virus, arenaviruses and hantaviruses.

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Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Matt Frieman

Matt joins Vincent, Alan, and Rich to summarize what we know and what we do not know about the MERS coronavirus.

• MERS-CoV update (WHO)
• MERS-CoV evolution (epidemic)
• Sick camel linked to MERS-CoV death (7Days)
• MERS-CoV hospital outbreak (NEJM)
• MERS-CoV French hospital cluster (Lancet)
• MERS-CoV family cluster (NEJM)
• Matt on MERS-CoV on the Voice of Russia
• Letters read on TWiV 239

Matt - The Kid Should See ThisAlan - Cassini to photograph Earth Vincent -Cockroaches! (YouTube)Rich - Sydney Brenner (Web of Stories)

Ebrahim - Virus Hunter by CJ Peters and Mark Olshaker

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Hosts: Vincent Racaniello, Rich Condit, and Kathy Spindler

Vincent, Rich and Kathy discuss mechanisms of protein synthesis and regulation in virus-infected cells.

• Slides for this episode (pdf)
• T7 ribosomal binding sites (CSH Symp Quant Biol)
• Tinkering with translation (CSH Persp)
• Translation at nexus of infection and immunity (Cell Host Microbe)
• Viral subversion of protein synthesis machinery (Nat Rev Micr)

Kathy - Vaccine-preventable outbreaksRich - To Catch a Virus by John Booss and Marilyn J. AugustVincent - This is Water by David Foster Wallace

Jon - Confessions of a converted lecturer by Eric MazurPeter - FameLab

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Hosts: Vincent Racaniello and Rich Condit

Guest: Michael Emerman

Vincent and Rich recorded this episode at the Fred Hutchinson Cancer Research Center in Seattle, WA, where they met up with Michael to talk about his work on the molecular and evolutionary basis of HIV replication and pathogenesis.

• Ancient origins of SIV (PLoS Path)
• Paleovirology (PLoS Biology)
• Harmit Malik on TWiV 205
• Sara Sawyer on TWiV 193

Molly - Facts in the case of Dr. Andrew Wakefield

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Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler

Vincent, Alan and Kathy review novel approaches to preventing influenza virus infection.

• Ultraprotective influenza vaccine in mice (PNAS)
• Vectored immunoprophylaxis of influenza in mice (Nat Biotech)
• Intranasal antibody gene transfer protects agains influenza (Sci Transl Med)
• Estimating influenza associated deaths (CDC)
• Multistate HAV outbreak (CDC)
• Subscribe to Microbeworld video (YouTube)
• Save podcasting (EFF)
• Letters read on TWiV 236

Alan - Psychology of antivaccinationistsVincent - Virology on CourseraKathy - Meteorites through the ages

Basel - Self-medication in animalsSheryl - Noadi's artFelicity - Survival of the Sickest by Sharon Moalem

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Hosts: Vincent Racaniello and Rich Condit

Guests: Dave Evans, Stan Lemon, and Lorne Tyrrell

Vincent and Rich recorded this episode before an audience at the 2nd Li Ka Shing Institute of Virology Symposium at the University of Alberta, where they spoke with Dave, Stan, and Lorne about their work on poxvirus vaccines and recombination, an enveloped picornavirus, antivirals against hepatitis B and C viruses, and supporting virology research in Alberta.

• Li Ka Shing Institute of Virology
• Alberta Heritage Foundation for Medical Research
• Raised on a farm (YouTube)
• Vaccinia virus strains in Dryvax vaccine (J Virol)
• Vaccinia DNA polymerase and recombination (J Virol)
• Picornavirus hijacks cell membrane (Nature)

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Hosts: Vincent Racaniello and Kathy Spindler

Guests: Nels Elde and Tom Shenk

Vincent and Kathy recorded this episode before an audience at the 2013 General Meeting of the American Society for Microbiology in Denver, Colorado, where they spoke with Nels and Tom about their work on the evolution of virus-host conflict and how viruses influence the cell metabolome.

• A cross-species view on viruses (Curr Op Virol)
• Pox has got a squeeze box (TWiV 198)
• Saturated fatty acids needed for CMV (PLoS Path)
• CMV and HSV metabolomes differ (PLoS Path)
• Video of this episode - view at YouTube

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Hosts: Vincent Racaniello, Rich Condit, Alan Dove, and Kathy Spindler

Vincent, Rich, Alan and Kathy review aerosol transmission studies of influenza H1N1 x H5N1 reassortants, H7N9 infections in China, and the MERS coronavirus.

• Aerosol transmission of H1N1 x H5N1 reassortants (Science)
• It's good science (virology blog)
• Viral engineering dangers (Nature)
• Colorblind web page filter
• Influenza H7N9 update (ProMedMail)
• Influenza H7N9 risk assessment (WHO, pdf)
• Influenza H7N9 confirmed cases (WHO)
• New coronavirus gets a new name (ScienceInsider)
• Novel coronavirus infection update (WHO)
• Coronavirus infections (WHO)
• Letters read on TWiV 233

Alan - Space Oddity (YouTube)Rich - Walk through giant colonVincent - Transport proteinsKathy - Vomiting Larry (YouTube)

Tom - US patent system is broken (DailyTech)

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Hosts: Vincent Racaniello, Roberto Cattaneo, Reuben Harris, Lou Mansky, and Leslie Schiff

Vincent meets up with Roberto, Reuben, Lou, and Leslie at the University of Minnesota to talk about their work on HIV-1, APOBEC proteins, measles virus, and teaching virology to undergraduates.

• University of Minnesota virology timeline
• Revisiting HIV-1 lethal mutagenesis (Trends Micro)
• Exploiting HIV-1 drug repositioning (J Virol)
• 5-azaC induces HIV-1 lethal mutagenesis (J Virol)
• HIV-1 fitness and mutation rate (J Mol Biol)
• APOBEC3 mutagenesis in breast cancer (Nature)
• Restriction factors of HIV (J Biol Chem)
• Nectin-4 is epithelial measles receptor (Nature)
• Photographs of this episode: Click here
• Video of this episode - YouTube

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Hosts: Vincent Racaniello, Amit Kapoor, Lan Quan, and Ian Lipkin

Vincent meets up with Amit, Lan, and Ian to discuss their discovery of hepaciviruses and pegiviruses in bats and rodents.

• Canine hepacivirus (TWiV 137)
• Rodent homologs of hepaciviruses and pegiviruses (mBio)
• Bats are a reservoir of hepaciviruses and pegiviruses (PNAS)

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Hosts: Vincent Racaniello, Rich Condit, Alan Dove, and Kathy Spindler

Vincent, Rich, Alan and Kathy review H7N9 infections in China, the debate over patenting genes, and receptor-binding by ferret-transmissible avian H5 influenza virus.

• Francois Jacob dies (The Loom)
• WHO updates on H7N9
• H7N9 is unusually dangerous (ScienceInsider)
• H7N9 case imported into Taiwan
• No H7N9 in poultry farms (Xinhua)
• Supreme Court considers gene patents (TheScientist, Nature)
• Patent my jeans, not my genes (Michael Wosnick)
• Receptor binding by ferret transmissible H5 virus (Nature)
• Microscale thermophoresis and bio-layer interferometry (Wikipedia)
• Letters read on TWiV 230

Alan - World Science FestivalRich - 3 years of the sun in 3 minutesVincent - The Origin of AIDS by Jacques PepinKathy - Wringing out a wet washcloth in space (YouTube)

Mark - Will it blend? (iPhone app)Marianne - Enjoy your Cells

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Hosts: Vincent Racaniello, Rich Condit, Dickson Despommier, and Alan Dove

Vincent, Rich, Dickson, and Alan review the current status of human infections with avian influenza H7N9 virus.

• Hilary Koprowski, 96 (NY Times)
• Koprowski, Kessin, Racaniello photo
• Listen to the Music by Roger Vaughan
• Influenza ocular infections (one, two)
• H7N9 global concerns (NEJM)
• Human infection with H7N9 (NEJM)
• WHO Global Alert and Response, H7N9
• Avian influenza A(H7N9) virus (WHO)
• Novel H7N9 reassortant, implications for Europe (Eurosurveill)
• Genetic analysis of H7N9 (Eurosurveill)
• H7N9 compared with H7N7 outbreaks (Eurosurveill)
• Image credit: Eurosurveillance
• Letters read on TWiV 229

Alan - Nature PastCastRich - How dengue does itVincent - Squeaky Squeegee Art by Deb SklutDickson - Agritecture

Mike - Chemistry in its element: Elements and Compounds

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Hosts: Vincent Racaniello, Britt Glausinger, and Eva Harris

Vincent visits the University of California at Berkeley and speaks with Britt Glaunsinger and Eva Harris about their work on Kaposi's sarcoma associated herpesvirus and dengue virus.

Thanks to the Microbiology Graduate Students for hosting me at their annual symposium, and especially to Emma, Lisa, and Zoe for their wonderful hospitality during my stay.

• RNA element in IL-6 confers SOX resistance (J Virol)
• Dual uORFs regulate herpesviral translation (PLoS Path)
• Common viral strategy for RNA degradation (J Virol)
• KSHV SOX and Xrn1 together degrade host mRNAs (PLoS Path)
• Dengue treatment with Fc null antibodies (PLoS Path)
• Early clinical features of dengue in Nicaraguan children (PLoS Negl Trop Dis)
• Intrahuman dengue virus diversity (J Virol)
• Dynamics of dengue disease severity (Sci Transl Med)

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Hosts: Vincent Racaniello, Rich Condit, Dickson Despommier, Alan Dove, and Kathy Spindler

The complete TWiV team reviews the controversial publication of the HeLa cell genome, a missing vial of Guanarito virus in a BSL-4 facility, and human infections with avian influenza H7N9 virus.

• Containment plan for poliovirus (pdf, WHO)
• Post-eradication of polio
• Ocular tropism of respiratory viruses (MMBR)
• Rally for medical research
• HeLa genome sequence withdrawn (ScienceInsider)
• Immortal life sequel (Rebecca Skloot)
• HeLa genome sequence published, withdrawn (virology blog)
• Vial of Guanarito virus missing (ABC news)
• Human H7N9 infections (ProMedMail)
• WHO H7N9 updates
• China escalates H7N9 response (NY Times)
• Brian Kimble on H7N9
• Compatibility of H9N2 and H1N1 genes (PNAS)
• Letters read on TWiV 227

Kathy - NIAID YouTube videosAlan - Lego insectsRich - How to see the world in a grain of sandVincent - Vaccines, viruses, and the anti-vax movementDickson - Edinburgh Science Festival

Stephen - Nature iOS appMehul - Voices for vaccines

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Hosts: Vincent Racaniello, Dickson Despommier, and Terry Dermody

Vincent and Dickson speak with Terry Dermody about his career in medicine and virology.

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Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler

Vincent, Rich, and Kathy read listener comments and questions on viral oncotherapy, science communication, a functional HIV cure in an infant, and much more.

• Plant viruses alter insect behavior (Sci Rep)
• Circovirus in Shanghai
• Letters read on TWiV 225

Kathy - Case stories about CoV-EMC (one, two)Rich - The Self-Illusion by Bruce HoodVincent - Moving Targets

Liam - eLIFE and RubriqSteve - Plant virus ecology (mp3)Neil - World Immunization Week

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Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler

Vincent, Alan, Kathy, and Dickson discuss identification of a cell receptor for the coronavirus-EMC, and the role of interferon-epsilon in protecting the female reproductive tract.

• Dipeptidyl peptidase 4 is CoV-EMC receptor (Nature)
• Receptor for new coronavirus (Nature)
• Broad reception for coronavirus (Nature)
• SARS 10th anniversary (Science)
• Interferon-epsilon protects female reproductive tract (Science)
• Families fighting flu (YouTube)
• Monitoring infections with online data (TWiM 41)
• Natalie Portman was a scientist (NY Times)
• Letters read on TWiV 223

Kathy - 2013 Intel Science Talent Search awardsAlan - Krebs Cycle Rap (CDC)Vincent - Jetpens (favorite one and two)D ickson - Rabies death in organ recipients (CDC)

Justin - The DNA StoreJim - Massive Open Online ClassroomsChris - Feedly

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Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler

Vincent, Alan, and Kathy discuss new influenza virus NA inhibitors, detection of EEEV antibody and RNA in snakes, and replication of the coronavirus EMC in human airway epithelial cells.

• International Women's Day 2013
• Condit Dam, 6 weeks later (Vimeo)
• Influenza HA assay
• Covalent influenza NA inhibitors (ScienceExpress)
• Defective influenza HA mutants (Virology)
• New flu drug (BBC, e! Science)
• EEEV antibody, RNA in snakes (Am J Trop Med Hyg)
• EEEV (CDC)
• Zoonotic potential of CoV-EMC (mBio)
• CoV-EMC update (ProMedMail, CDC)
• CoV-EMC travel update (CDC)
• Letters read on TWiV 223

Kathy - Finkbeiner test and Women in ScienceAlan - New flu vaccine efficacy study (CDC)Vincent - Threading the NEIDL video

Steve - Prof. Joan Steitz on viral RNAEd - Archiving history of molecular biology: Press release, video, CSHL archives and videos

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Hosts: Vincent Racaniello, Rich Condit, Dickson Despommier, Alan Dove, and Kathy Spindler

The complete TWiV team discusses the amazing finding that cyclic GMP-AMP synthase is a cytosolic innate immune DNA sensor.

• Zhijian J. Chen (Wikipedia)
• Sensing the dark side of DNA (Science)
• cGAMP is an endogenous second messenger (Science)
• cGAMP synthase is a DNA sensor (Science)
• Pore-forming toxin (Wikipedia)
• Letters read on TWiV 222

Kathy - Graphene batteriesAlan - How do we get cures? (YouTube)Rich - Explosive breach of Condit Dam (Vimeo)Vincent - 10 things you can do to support open access (Tree of Life)

Mark - The real story of StuxnetJohn - D-connector virus

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Hosts: Vincent Racaniello, Dickson Despommier, and Kathy Spindler

Vincent, Dickson, and Kathy review two emerging bunyaviruses, SFTSV and SBV.

• Human transmission of SFTSV (JID)
• SFTSV serosurvey (EID)
• SFTSV on TWiV 147
• SFTSV in Japan (ProMedMail)
• SFTSV mouse model (PNAS)
• SBV infectious DNA and mouse model (PLoS Path)
• SBV in Sweden (ProMedMail)
• Letters read on TWiV 221

Kathy - Winners, 2012 Science & Engineering Visualization ChallengeVincent - The Operator and Dr. Oz's Problem

Shane - OpenPCRJohn - Python

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Hosts: Vincent Racaniello, Rich Condit, Alan Dove, and Kathy Spindler

Vincent, Rich, Alan, and Kathy discuss regulation of influenza virus replication by splicing, and the bacteriophage T7 random walk.

• Influenza splicing regulates infection (Cell Reports)
• Reporting on flu splicing from NPR, BBC, NBC
• Splicing of influenza RNA 8 (jpg)
• Bacteriophage T7 remodeling during infection (Science)
• Cryo-ET of intact cells (Trends Cell Biol)
• T7: The Movie (YouTube)
• Cry0-EM vs Cryo-ET (Wikipedia)
• Virus caught in the act (e! Science News)
• Letters read on TWiV 220

Kathy - Journal of Virology Image GalleryRich - Phage T7 Genetics and Physiology and slab gelsAlan - PeerJ (press release)Vincent - Virologia en Español

Dave - Thingiverse pathogens and proteins

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Hosts: Vincent Racaniello, Rich Condit, and Anthony S. Fauci

Vincent and Rich meet up with Anthony S. Fauci, MD, Director of the National Institute of Allergy and Infectious Diseases.

• Fauci history (J Clin Inves)
• Fauci Pharmacy
• Anthony Fauci (Wikipedia)
• Anthony Fauci: Unfinished business (Lens)

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Hosts: Vincent Racaniello, Alan Dove, and Welkin Johnson

Vincent, Alan, and Welkin discuss how endogenous retroviruses in mice are held in check by the immune response.

• Kuan-Teh Jeang, 54
• Resurrection of ERVs (Nature)
• Toll-like receptors control ERVs (Immunity)
• Retroviral danger from within (Immunity)
• Ancient viruses wreak havoc (The Scientist)
• Letters read on TWiV 218

Welkin - Viruses: Essential Agents of Life (review at STC)Alan - Infinity ImaginedVincent - Spaceship built for son

Amanda - Written? Kitten!Kevin - Protein synthesis: An epic (YouTube)Daria - The Baltimore Scheme

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Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Dickson Despommier

Vincent, Alan, Rich, and Dickson review influenza vaccines.

• FDA licensed flu vaccines
• Seasonal influenza vaccine distributed
• How flu vaccine strains are selected (one, two)
• WHO report on flu vaccine strain selection (pdf)
• Derek Smith on antigenic mapping
• Salk's 1945 influenza vaccine
• TIV preparation: Fluzone, Agriflu, Fluvirin, Fluarix, Flulaval, Afluria (pdfs)
• LAIV preparation (pdf)
• Efficacy of flu vaccine 2012-13 (MMWR, virology blog)
• Meta-analysis of flu vaccine efficacy (Lancet)
• Two new flu vaccines FDA approved
• Flublok
• Flublok clinical trial
• Pandemrix and narcolepsy (Yahoo, PLoS One, PLoS One)
• CIDRAP analysis of flu vaccines
• Letters read on TWiV 217

Rich - Repoopulate (original article), Robogut (new clinical trial)Alan - WTF, Evolution?Dickson - The Beauty and Benefits of ScienceVincent - Supercomputing modeling of poliovirus

Bjorn, Marshall, Meaghan - Glass sculptures of Luke Jerram

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Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler

Vincent, Alan, Rich, and Kathy resume the virology 101 series with a discussion of RNA capping, splicing, and export.

• Slides for this episode (pdf)
• Spliced Ad 2 late mRNAs (Cell)
• An amazing sequence arrangement (Cell)
• Adenovirus late mRNA undecanucleotide (Cell)
• Aaron Shatkin, 72 (virology blog)
• Schrödinger's cat (Wikipedia)
• Letters read on TWiV 216

Rich - Linus Pauling's explanation of science (YouTube)Alan - Underwater experimentsKathy - I'm a virus (YouTube)Vincent - Stem cells (Bizarro Comics)

Tom - The President's AnalystDanielle - Overly honest methods (HuffPost and ASBMB Today)

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Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler

Vincent, Alan, and Kathy review the finding that rabies virus infection alters but does not kill neurons, and provide an update on the novel coronavirus in the Middle East.

• Neuronal survival after rabies virus infection (PLoS Path)
• Clusters of coronavirus cases (Nature)
• ProMedMail on novel coronavirus
• Novel coronavirus outbreak news (Int J Inf Dis)
• Genomic characterization of novel coronavirus (mBio)
• Broad cell tropism of novel coronavirus (mBio)
• Are viruses alive? (poll at virology blog)
• Antibodies taken up by endocytosis (PNAS)
• Antibody eliminates alphaviruses from neurons (Science)
• Image: Virus in the garden by Helen Hache
• Letters read on TWiV 215

Alan - I'm a Scientist - The FilmKathy - Humorous units of measurement (Wikipedia)Vincent - Open access must enable open use (Nature)

Tarwin - Plague Inc.

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Hosts: Vincent Racaniello, Rich Alan Dove, and Kathy Spindler

Vincent, Alan, and Kathy discuss how coagulation factor X binding to adenovirus activates the innate immune system, and a novel polyomavirus associated with brain tumors in raccoons.

• Coagulation factor X activates innate immunity to adenovirus (Science)
• A decorated virus cannot hide (Science)
• Reflecting on goals for Science
• Polyomavirus associated with raccoon brain tumors (EID)
• MPNST
• Moore tumor viruses - TWiV 160
• Treating CLL with lentivirus vectors (NEJM)
• Letters read on TWiV 214

Alan - Parasite of the DayKathy - Tour of International Space StationVincent - Earth as Art

Robin - Angell on Big PharmaMichael - Flu jokesJim - Database of Ted talks; Downloadable Ted Talks

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Hosts: Vincent Racaniello, Rich Condit, Alan Dove, and Kathy Spindler

In their final episode of the year, the TWiV team reviews twelve cool virology stories from 2012.

1. H5N1 (TWiV 190, 182, 177, 173, 168)
2. Polymorphisms that control susceptibility (TWiV 175, 180)
3. New virus receptors (TWiV 166, 210)
4. XMRV: Last nail in the coffin (TWiV special)
5. Polio eradication troubles in Pakistan (NY Times one, two, three; virology blog)
6. Biomedical research crisis (TWiV 208, 184, 189, 194)
7. Polydnaviruses in parasitoid wasps (TWiV 179)
8. Carolyn Coyne’s placental barrier story (TWiV 193)
9. Touring NEIDL (TWiV 200)
10. Epidemiology (TWiV 169, 178)
11. A good year for virus hunting (173, 183, 195, 196, 198, 199, 204)
12. Pox accordion (TWiV 198)

• US plans for H5N1 research (ScienceInsider)
• FDA recommends against Ampligen
• Rituximab and CFS (PLoS One)
• No evidence for XMRV in prostate cancer (PLoS One)
• Retraction of XMRV prostate cancer paper (Retraction Watch)
• Gender bias among science faculty (PNAS)
• NEIDL risk assessment
• Virology 101 at TWiV
• Letters read on TWiV 213

Rich - Our Mr. Sun (IMDB entry) (wiki)Alan - PubReader from NCBI announcement and instructionsKathy - Popular Mechanics 110 picks for the next 110 yearsVincent - 366 days: Nature's 10

Matt - The flu vaccine controversy

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Hosts: Vincent Racaniello and Stuart Firestein

Vincent and Stuart discuss why ignorance - all of what we don't know, and even what we don't know we don't know - is the driving force of science.

• Ignorance: How it drives Science by Stuart Firestein
• Ignorance website

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Hosts: Vincent Racaniello, Rich Condit, Alan Dove, and Kathy Spindler

The TWiVerers answer listener email about genetically modified chickens, a hendra vaccine for horses, online education, curing color blindness, Roosevelt and polio, Th cells, and much more.

• TWiV 116: Cocaine, colonies, and chickens
• Glofish
• Recombinant Hendra vaccine
• Gene therapy for color blindness (Nature)
• FDR, polio, and Guillain-Barré
• Transcript of TWiV 197: Philip Marcus (pdf)
• TWiV 10: Bats, elephants, and AIDS
• TWiM 6: Phage therapy
• TWiP 47: For whom the trich tolls
• Viruses of protozoan parasites (virology blog)
• Long in the tooth
• Transcript of 'How to read a science paper' (pdf)
• FEMA disaster preparedness courses
• Letters read on TWiV 212

Rich - OverviewAlan - Notes towards the complete works of ShakespeareKathy - Correlation vs. Causation (#1, #2, #3)Vincent - Periodically inspired

Mark - Life of a Nature paperRicardo - Things don't go viral

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Hosts: Vincent Racaniello, Rich Condit, Alan Dove, and Kathy Spindler

The TWiV four discuss an mRNA-based influenza vaccine, and a phage tubulin that forms a filamentous array in the host cell that is needed for positioning viral DNA.

• mRNA vaccine against influenza (Nat Biotech)
• Phage tubulin assembles dynamic filaments (Cell)
• Viral skeleton (The Scientist)
• Not unorganized bags of enzymes (TWiM 28)
• Bacterial cytoskeleton (FEMS Micro Rev)
• Triangulation number (jpg)
• Letters read on TWiV 211

Rich - Ocean Global Shark TrackerAlan - The Field Book ProjectKathy - How to manipulate an army of zombiesVincent - Science sculpture

Stephen - WEHI.TV

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Hosts: Vincent Racaniello, Rich Condit, Dickson Despommier, Alan Dove, and Kathy Spindler

The complete TWiV team reviews identification of the cell receptor for hepatitis B and D viruses, and the cell enzyme that cleaves the genome-linked protein from picornaviral RNA.

• HBV and HDV cell receptor (eLIFE)
• Getting to grips with hepatitis (eLIFE)
• Sodium taurocholate
• Poliovirus hijacks DNA repair enzyme (PNAS)
• Letters read on TWiV 210

Rich - Vendée GlobeDickson - Earth at nightAlan - Trophée Jules VerneKathy - Science cookiesVincent - Trilobite Glassworks

Ken - Spanish flu pandemic model (teacher's guide)Jim - Santa Cruz Science Communication Program (also see SHERP)

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler

Vincent, Dickson, Alan, and Kathy answer listener email about deformed wing virus, West Nile virus, FluMist, influenza in Canada, viruses and the tree of life, and more.

Links for this episode:

• Postcard from the ultimate student athlete (pdf)
• Fidelity of protein synthesis (Biochemistry, 5th ed.)
• Letters read on TWiV 209

Dickson - Cassini web site and Mars reconnaissance orbiterAlan - OneZoom Tree of LifeKathy - Viral tumor suppressor structure (Cell)Vincent - Spot the Station

Ricardo - Once upon a time: The possible story of virusesJim - Encode, myIDP, Experiment Reproduction podcastsMark - MoMath

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Hosts: Vincent Racaniello and Jon Yewdell

Vincent and is joined by special guest Jon Yewdell to discuss solutions for ending the current crisis in American biomedical research.

• How to succeed in science: Taking the plunge (Nat Rev Mol Cell Biol)
• How to succeed in science: Making discoveries (Nat Rev Mol Cell Biol)
• How to succeed in science (YouTube)
• Resources for graduate students and postdocs
• Science career cartoons by Alexander Dent

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Hosts: Vincent Racaniello, Alan Dove, Matt Frieman, and Kathy Spindler

Vincent, Alan, Matt, and Kathy review the use of silk to stabilize antibiotics and a viral vaccine, and an impaired-fidelity vaccine against SARS coronavirus.

• Silk stabilization of antibiotics and vaccines (PNAS)
• Impaired-fidelity coronavirus vaccine (Nature Medicine)
• Select agent status and SARS therapies (Spoonful of Medicine)
• Letters read on TWiV 207

Matt - Virus-associated pyramids (slide #11)Alan - Similarities of wave behavior (YouTube)Kathy - The glass transitionVincent - US cattle country in uproar at virus lab plans (BBC)

Ricardo - Blackawton bees and Science is for everyone (TED)Tony - Biophilia by Bjork

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv

Hosts: Vincent Racaniello, Alan Dove, Dickson Despommier, and Kathy Spindler

Vincent, Alan, Dickson, and Kathy discuss how the innate immune response to viral infection influences the production of pluripotent stem cells, and the diverse mobilome of giant viruses.

• Innate immunity and nuclear reprogramming (Cell)
• Viruses affect cell reprogramming (The Scientist)
• Cell-penetrating peptides (Trends Mol Med)
• Provirophages and transpovirons (PNAS)
• Organic Lake photo and map
• Letters read on TWiV 206

Alan - Locust by Jeffery A. LockwoodDickson - Nikon Small World Contest 2012Kathy - Influenza EncyclopediaVincent - Dengue fever sweeps India (NY Times)

Allison - Fred Guterl on The Daily Show

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv.

Hosts: Vincent Racaniello and Harmit Malik

Vincent is joined by special guest Harmit Malik to discuss his work on the evolution of genetic conflict.

• Harmit on YouTube
• Retroviruses and mammalian placentation (PNAS)
• Paleovirology (Curr Opin Virol)
• Evolution among AIM2-like receptors (J Exp Med)
• Poxvirus genomic accordions (Cell)
• Letters read on TWiV 205

Vincent - Candidates' answers to science questions

Steve - Mural of Henrietta Lacks story (Microsoft Silverlight required)

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv. You can also post articles that you would like us to discuss at microbeworld.org and tag them with twiv.

Hosts: Vincent Racaniello, Alan Dove, Matt Frieman, and Kathy Spindler

Vincent, Alan, Matt and Kathy review isolation of a new coronavirus from two patients in the Middle East, and expansion of the enteric virome during simian AIDS.

Subscribe to TWiV (free) in iTunes, at the Zune Marketplace, by the RSS feed, by email, or listen on your mobile device with the Microbeworld app.

• Novel coronavirus (virology blog one, two)
• PCR assay for novel CoV (Eurosurveillance)
• Isolation of novel coronavirus (NEJM)
• SARS CoV now a select agent (Federal Register, pdf)
• My Sharona
• Pathogenic SIV infection associated with expansion of gut virome (Cell)
• Letters read on TWiV 204

Alan - Map of the WindMatt - Dark Side of the Earth; Insensitive guide to thriving in your Ph.D.; Gary Vanerchuck (YouTube)Kathy - How much does a shadow weigh? and Slinky drop answerVincent - A Parasite's Parasites; All the cool kids are on arXiv

Nissin - Jungle Jim (1948)

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv. You can also post articles that you would like us to discuss at microbeworld.org and tag them with twiv.

Hosts: Vincent Racaniello, Rich Condit, and Mark Challberg

Vincent and Rich meet up with Mark Challberg to talk about his scientific career studying viral DNA replication, and his transition to an NIH Program Officer.

Subscribe to TWiV (free) in iTunes, at the Zune Marketplace, by the RSS feed, by email, or listen on your mobile device with the Microbeworld app.

• Purification of vaccinia virus DNA polymerase (J Biol Chem)
• Adenovirus DNA replication in vitro (PNAS)
• Herpes simplex viral genes required for DNA synthesis (J Virol)
• Letters read on TWiV 203

Rich - Mattel Hot Wheels Curiosity RoverVincent - Should H5N1 Moratorium End?

Claudio - DIY lab equipment (Ars Technica) and DIY Bio

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv. You can also post articles that you would like us to discuss at microbeworld.org and tag them with twiv.

Hosts: Vincent Racaniello, T. Jack Morris, James Van Etten, and Charles Wood

Vincent travels to the University of Nebraska to meet with members of the Nebraska Center for Virology and discuss their work on algal viruses, plant viruses, HIV and Kaposi's sarcoma herpesvirus.

• The Nebraska Center for Viology
• Behind the scenes at TWiV 202
• TWiV on Facebook

Charles - Rhabdovirus associated with hemorrhagic fever (PLoS Path)Vincent - Bacteriophage graffiti (jpg)

Bob - Remembering Penny PinneoJanet - Penn and Teller on vaccinations (YouTube)

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv. You can also post articles that you would like us to discuss at microbeworld.org and tag them with twiv.

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler

Vincent, Alan, Rich, and Kathy answer reader email about rabies, xenotransplantation, poliovirus, Ph.D. programs, mosquitoes, and much more.

• Mattias Schnell
• Novel rhabdovirus from Africa (Plos Path)
• Milwaukee protocol (Wikipedia)
• How lethal is rabies virus? (virology blog)
• Issues with polio eradication (J Gen Virol)
• Vaccine-derived polioviruses (J Inf Dis)
• Global pandemics with Hidde Ploegh (Vimeo)
• Polio vs Bin Laden (Alan Dove)
• Letters read on TWiV 201

Alan - Wallace OnlineRich - World Rabies DayKathy - Yo-yos in spaceDickson - The Art in ScienceVincent - The Podcasters Studio

Wink - DuckDuckGoJosh - Introduction to Solid State Chemistry

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv. You can also post articles that you would like us to discuss at microbeworld.org and tag them with twiv.

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, Elke Mühlberger, Paul Duprex, and Ron Corley

Vincent, Alan, and Rich celebrate the 200th episode of TWiV by visiting the National Emerging Infectious Diseases Laboratories at Boston University Medical Center, where they meet with Elke, Paul, and Ron to talk about building and working in a BSL4 facility.

• The NEIDL
• NEIDL goes public (BU Today)
• Biosafety levels (Wikipedia)
• Select agents (Wikipedia)
• NEIDL stalled (Boston Business J)
• TWiV on Facebook
• Letters read on TWiV 200

Alan - Dance your Ph.D.Rich - Angel of Death by Gareth WilliamsVincent - Why herpesvirus is good for you (Virgin lab)

Luis - VBORNET maps

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv, or call them in to 908-312-0760. You can also post articles that you would like us to discuss at microbeworld.org and tag them with twiv.

Hosts: Vincent Racaniello and W. Ian Lipkin

Vincent and Ian review a multicenter blinded analysis which finds no association between chronic fatigue syndrome/myalgic encephalomyelitis and XMRV or polytropic murine leukemia virus.

Subscribe to TWiV (free) in iTunes, at the Zune Marketplace, by the RSS feed, by email, or listen on your mobile device with the Microbeworld app.

• Multicenter blinded analysis of CFS and XMRV/pMLV (mBio)
• Press release on multicenter study (pdf)
• Press conference webcast
• TWiV on Facebook

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv, or call them in to 908-312-0760. You can also post articles that you would like us to discuss at microbeworld.org and tag them with twiv.

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler

Vincent, Alan, Rich, and Kathy discuss recent outbreaks of hantavirus pulmonary syndrome in Yosemite National Park and novel swine-origin influenza in the US midwest, and isolation of the Heartland virus from two patients in Missouri with severe febrile illness.

Subscribe to TWiV (free) in iTunes, at the Zune Marketplace, by the RSS feed, by email, or listen on your mobile device with the Microbeworld app.

• Hantavirus outbreak in Yosemite (ProMedMail)
• Hantavirus in Yosemite (CDC)
• CDC page on hantavirus
• Summary of hantavirus outbreak (Avian Flu Diary)
• Swine-origin influenza outbreak in midwest (MMWR)
• Lyn Finelli interview on swine-origin H3N2 (Microbeworld)
• Transmission of vH3N2 in ferrets (PNAS)
• New phlebovirus from Missouri (NEJM)
• TWiV on Facebook
• Letters read on TWiV 199

Alan - Insane in the Chromatophores (Vimeo)Rich - Golden Goose AwardsKathy - Euler's Disk (YouTube)Vincent - Ignorance: How it Drives Science by Stuart Firestein

Adam - Leigh Van Valen

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv, or call them in to 908-312-0760. You can also post articles that you would like us to discuss at microbeworld.org and tag them with twiv.

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler

Vincent, Alan, Rich, and Kathy review fatal avian influenza virus in harbor seals, and poxvirus deployment of genomic accordions to counter antiviral defenses.

• Avian influenza virus in harbor seals (mBio)
• Poxvirus genomic accordions (mBio)
• Red Queen hypothesis (Wikipedia)
• TWiV on Facebook
• Letters read on TWiV 198

Alan - Getting Better: 200 Years of MedicineRich - Khan AcademyKathy - Vi HartVincent - Tweet Keeper

Diane - The Way We Fall by Megan CreweStephen - Amateur Micrography

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv, or call them in to 908-312-0760. You can also post articles that you would like us to discuss at microbeworld.org and tag them with twiv.

Hosts: Vincent Racaniello and Philip I. Marcus

Vincent travels to the University of Connecticut to meet up with Professor Philip I. Marcus to discuss his development of the single cell cloning technique in the early 1950s.

• Cloning with X-irradiated feeder cells (PNAS)
• Philip I. Marcus in the lab (jpg)
• Cloning with capillary tubes (jpg)
• Cloning platform (jpg)
• VRR holding 1950s glass plate of clones (jpg)
• HeLa clones (jpg)
• Cloning cylinder (jpg)
• TWiV on Facebook
• Coming soon: video of this episode

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv, or call them in to 908-312-0760. You can also post articles that you would like us to discuss at microbeworld.org and tag them with twiv.

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, Dickson Despommier, Kathy Spindler, Mark Stenglein, and Joseph DeRisi

The TWiVites meet with Mark Stenglein and Joseph DeRisi to discuss their discovery of a novel arenavirus in snakes with inclusion body disease.

• Highly divergent arenaviruses in snakes (mBio)
• Taryn's sick snake (NPR)
• Assemblathon
• Faculty position in virology, Columbia U.
• Petridish.org
• TWiV on Facebook
• Letters read on TWiV 196

Mark - The Slow Mo Guys (Fly eats fly)Dickson - I'm being eaten by a boa constrictor by Shel SilversteinAlan - MMWR: First 30 years onlineRich - Giant python in Everglades and California Academy of SciencesKathy - Darwin finch genome sequenceVincent - Microbiology at Mt. Sinai

Renato - Security Now podcastRobin - The WeatherChad - Rare protozoan from sludge (ScienceDaily)

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv, or call them in to 908-312-0760. You can also post articles that you would like us to discuss at microbeworld.org and tag them with twiv.

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, Dickson Despommier, and Ken Stedman

The complete TWiV team meets with Ken Stedman to discuss the discovery in Boiling Spring Lake of a DNA virus with the capsid of an RNA virus.

Subscribe to TWiV (free) in iTunes , at the Zune Marketplace, by the RSS feed, by email, or listen on your mobile device with the Microbeworld app.

• Novel virus from an extreme environment (Biol Direct, virology blog)
• Lassen Volcanic National Park
• Tombusviridae, Circoviridae (ViralZone)
• TWiV on Facebook
• Letters read on TWiV 195

Ken - The Edge of Life trailer and Facebook pageDickson - The Cognitive Style of Powerpoint by Edward TufteAlan - SEMs of insects and spiders via Steve GschmeissnerRich - Curiosity Has Landed (Wiki mission summary)Vincent - Curiosity's Seven Minutes of Terror (YouTube)

Lance - The Vaccine Confidence Project and Immune response video (YouTube)Anat - Beautiful Science

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv, or call them in to 908-312-0760. You can also post articles that you would like us to discuss at microbeworld.org and tag them with twiv.