This Week in Virology

Hosts: Vincent Racaniello, Alan Dove, and Rich Condit

Guests: David Pride and Forest Rohwer

If you have always wanted to know what coral reefs and the human oral cavity have in common, listen as guests David Pride and Forest Rohwer talk about their work on the microbiomes and viromes of these two environments, and you'll also understand why mucus is cool.

• Lytic to temperate switching of viral communities (Nature)
• Viromes in ancestral holobiont Hydra (PLoS One)
• Phage in human oral cavity (J Oral Micro)
• Human oral viruses are personal, consistent, gender specific (ISME)
• Video of this episode at YouTube

This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE.

Alan - Vax Pack HeroRich - Helicopter autorotation Vincent - Live in Our Phage World by Forest Rohwer, Merry Youle, Heather Maughan, Nao Hisakawa

Matt - Experiment.com

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler

Guests: Ethan Jackson and Jonathan Carlson

Project Premonition, a Microsoft Research project that uses drones to capture mosquitoes and analyze them for pathogens, preprint servers, and three mouse models for Zika virus induced birth defects are the topics of this episode hosted by the TWiumvirate and theirs guests Ethan and Jonathan.

• Project Premonition
• Premonition feasibility study in Grenada
• Zika virus causes microcephaly in mice (Cell Stem Cell)
• Brazilian Zika virus causes defects in experimental models (Nature)
• Zika virus causes placental damage and fetal demise in mice (Cell)
• Letters read on TWiV 390

This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE.

Also brought to you by ASV 2016

Dickson - Germany nearly reached 100% renewable power Alan - Vertical City documentary and Lumino City gameRich - Errol Morris: 'Demon in the Freezer' Kathy - Entropic Time YouTube by A Capella Science (watch "the making of")Vincent - Agar Art contest finalists

Jennie - SciFi reading (scroll down)

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Host: Vincent Racaniello

Guest: Stan Lemon

Vincent speaks with Stan Lemon about his career in virology, from early work on Epstein Barr virus, through making essential discoveries about hepatitis A virus, hepatitis C virus, and rhinoviruses.

• Live in Edmonton, Eh? (TWiV 235)
• Replication of EBV in epithelial cells (Nature)
• Returning to Carolina after 14 years
• Evolutionary origins of hepatitis A virus (TWiV 265)
• cre in rhinovirus genome (RNA)
• Naked viruses that aren't always naked (Ann Rev Virol)
• miR-122 and hepatitis C virus RNA (Cell Host Micr)

This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE.

Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler

Preprint servers, the structure of an antibody bound to Zika virus, blocking Zika virus replication in mosquitoes with Wolbachia, and killing carp in Australia with a herpesvirus are topics of this episode hosted by Vincent, Dickson, Alan, and Kathy.

Links for this episode

• biorXiv preprint server
• Please review Vaccines and Your Child
• Structure of antibody bound to Zika virus E protein (Cell Host Microbe)
• Wolbachia blocks Zika virus in mosquitoes (Cell Host Microbe)
• Zimmer on Wolbachia and Zika virus (NY Times)
• Fauci on Zika virus vaccine (Science)
• Carpageddon (The Conversation)
• Letters read on TWiV 388

This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE.

Also brought to you by ASV 2016

Dickson - EarthEnv Alan - Research funding by lottery Kathy - Eugenia Cheng Math and BakingVincent - Zika Diaries

Stephen - Virus trading cards William - Virus trading cards Norma+Maurice - Virus trading cards Tom - Virus trading cards

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler

Guest: Nina Martin

Nina Martin joins the TWiV team to talk about the movie Vaxxed, her bout with dengue fever, and the latest research on Zika virus.

• Public Health United
• Fifty Shades of Science (TEDx)
• Parents convicted in death of toddler (Science Based Medicine)
• CDC: Zika virus causes microcephaly (NEJM)
• Dengue virus antibodies enhance Zika virus infection (biorXiv)
• Zika virus in Brazilian nonhuman primates (biorXiv)
• Zika virus IgM in CSF of microcephalic babies (Lancet)
• Brain organoids for modeling Zika virus infection (Cell)
• Letters read on TWiV 387

This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE.

Also brought to you by ASV 2016

Nina - Vaccines and Your Child by Paul Offit and Charlotte MoserDickson - The animals of Chernobyl Alan - Five rules of lab safety Kathy - 20 best science images of the year?Vincent - Massive undersea crab swarm

JP - Global map of wind Todd - Doc Martin

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Hosts: Vincent Racaniello, Rich Condit, and Kathy Spindler

Guests: Ted Diehl and Welkin Johnson

Ted and Welkin inform the TWiV team how the evolution of ancient retroviruses can be inferred by studying their sequences in the genomes of modern mammals, and join in a discussion of virus dispersal during different methods for drying hands.

• More fun than a monkey full of viruses (TWiV 122)
• Tracking ancient retrovirus evolution in mammals (eLife)
• Virus dispersal during hand drying (J Appl Micro)
• More than you wanted to know about hand drying (one, two, three, four, five, six)

This episode is sponsored by ASM Agar Art Contest, ASM Grant Writing Course, and ASV 2016

Welkin - Lab Girl by Hope Jahren (essay)Ted - Accidental brilliance in scienceRich - Perpetual motion?Kathy - R0 graphic and explanationVincent - NWS ditches all caps forecasts

Basel - Gimli Glider and Mars Climate OrbiterKen - Vaccination comic book

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Hosts: Vincent Racaniello and Dickson Despommier

Guest: Stuart Firestein

Stuart joins Vincent and Dickson to talk about his sequel to Ignorance, a book called Failure, which seeks to make science more appealing by revealing its faults.

• Stuart Firestein's site
• Ignorance with Stuart Firestein (TWiV Special)
• The pursuit of ignorance (TED)
• Ignorance by Stuart Firestein
• Failure by Stuart Firestein

This episode is sponsored by ASM Agar Art Contest and ASV 2016

Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove and Rich Condit

Guests: Nischay Mishra and Ian Lipkin

Nischay and Ian join the TWiVions to discuss mass die-offs of tilapia by a novel orthomyxo-like virus, Ian's editorial on the movie Vaxxed, and new vaccines to prevent dengue virus infections, including a human challenge model.

• Novel orthomyxo-like virus in Tilapia die-offs (mBio)
• Fish flu (TWiV 41)
• Anti-vaccination lunacy won't stop (WSJ)
• Single-dose dengue vaccine effective (J Inf Dis)
• Dengue vaccine needs (J Inf Dis)
• Composition of TV003 (J Inf Dis)
• Dengue vaccine mutagenesis (Curr Top Micr Imm)
• Dengue human challenge model (Sci Transl Med)
• Yellow fever alarm (STAT)
• Experiment: Zika virus
• UC Berkeley Microbiology Student Symposium
• Image credit
• Letters read on TWiV 384

This episode is sponsored by ASM Agar Art Contest, ASM Grant Writing Course, and ASV 2016

Rich - Dune by Frank HerbertAlan - Adult vaccination video contestDickson - Ski town greenhouseVincent - Preprint servers: Force awakens or revenge of undead?

Ken - Golden Mole Awards for Accidental Brilliance (list, video)Jim - Linear algebra course

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove and Kathy Spindler

Guest: Esper Kallas

Esper and the Merry TWiXters analyze the latest data on Zika virus and microcephaly in Brazil, and discuss publications on a mouse model for disease, infection of a fetus, mosquito vector competence, and the cryo-EM structure of the virus particle.

• Zika virus in the Americas (Science)
• Co-circulation of dengue, Zika, chik in Brazil (Medicine)
• Murine model for Zika virus infection (Am J Trop Med Hyg)
• Zika virus susceptibility of A. aegypti and A. albopictus (PLoS NTD)
• Infectious Zika virus from saliva (EuroSurveill)
• Zika virus spread through saliva? (Braz Oral Res)
• Five Zika virus reviews (one, two, three, four, five)
• Zika virus and microcephaly, Brazil (MMWR)
• Zika virus in urine (EuroSurveill)
• Zika virus infection of fetus (NEJM)
• Real-time sharing of Zika virus data (JAMA)
• Image credit: Richard Kuhn
• Letters read on TWiV 383

This episode is sponsored by ASM Agar Art Contest and ASV 2016

Esper - The Chaos by Gérard Nolst Trenité and HIV pathogenesis courseAlan - Science KombatDickson - Four steps to wiping out a mosquitoVincent - Public lacks information on Zika virusKathy - Zika virus cryo-EM structure video

Justin - Culture-independent discovery of Archaeal virusMelinda - Zika virus open research portal and Your body battles series

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove and Kathy Spindler

Guests: Nels Elde and Edward Chuong

Nels and Ed join the TWiV team to talk about their observation that regulation of the human interferon response depends on regulatory sequences that were co-opted millions of years ago from endogenous retroviruses.

• Endogenous retroviruses and regulation of innate immunity (Science)
• Placenta is viral (Nature)
• An ERV that maintains pluripotency of human embryonic stem cells (TWiV 279)
• Letters read on TWiV 382

This episode is sponsored by ASM Agar Art Contest and ASV 2016

Nels - Don't Get Me Started - Matt GiorgianniAlan - Sun's magnetic field (paper)Dickson - Most realistic dinosaur terrifiesVincent - Top secret!Kathy - Zika virus risk for US 50 cities (paper)Ed - Watching comb jellies poop

Asal - Lost paper of Gregor MendelDennis - PhysicsGirlJacob - Second (XKCD) and ACI blogger interview

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler

Guest: Carl Zimmer

Carl Zimmer joins the TWiV team to talk about his career in science writing, the real meaning of copy-paste, science publishing, the value of Twitter, preprint servers, his thoughts on science outreach, and much more.

• Carlzimmer.com
• All Carl's articles at NYTimes
• Carl at Stat
• The Loom
• Staying afloat in the rising tide of science (Cell)
• Parasite Rex
• Planet of Viruses
• Handful of biologists went rogue (NYTimes)

This episode is sponsored by ASV 2016 and ASM Agar Art Contest

Carl - Surprising benefit of viral DNAAlan - Global ship trafficDickson - Music with 2000 marblesVincent - The whiteboard revolutionKathy - How to interpret UK and American advisorsRich - Carina Storrs, science writer

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler

The TWiVeroos deliver the weekly Zika Report, then talk about a cryoEM structure of a plant virus that reveals how the RNA genome is packaged in the capsid, and MIMIVIRE, a CRISPR-like defense system in giant eukaryotic viruses.

• First worms, then viruses, now dormant cyber pathogens (Zdziarski blog)
• Guillain-Barré with Zika virus in urine (Eurosurveillance)
• Zika virus in urine, Brazil (J Clin Virol)
• Zika in pregnant women in Rio (NEJM)
• Issues with NEJM pregnant women study (Epi-Ramble)
• Zika virus and meningoencephalitis (NEJM)
• Zika virus and acute myelitis (Lancet)
• Person to person transmission of Zika virus (MMWR)
• Pyrethroids and mouse microcephaly (one, two)
• Zika virus illustrations (Visual Science)
• Cowpea mosaic virus at high resolution (Nat Comm)
• MIMIVIRE confers resistance to virophage (Nature)
• CRISPR in a phage (TWiM 56)
• National Academy of Sciences GoFoc meeting
• Image credit
• Letters read on TWiV 380

This episode is sponsored by ASM Agar Art Contest and Microbe Magazine Podcast

Alan - Progression of EvolutionDickson - Toddler dies of meningitisVincent - Gardasil ideology, not scienceKathy - The Noun ProjectRich - Physics Girl

Mark - MyRadar app (iOS, Android)

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler

Guest: Scott Tibbetts

Scott Tibbetts joins the TWiVists to describe his work on the role of a herpesviral nocoding RNA in establishment of peripheral latency, and then we visit two last minute additions to the Zika virus literature.

• Noncoding RNA required for establishment of latency (mSphere) 17:20
• Lessons learned from in vivo studies of a viral noncoding RNA (mSphere) 1:05:05
• Zika virus in pregnant women in Rio de Janeiro (NEJM) 1:06:10
• Zika virus infects cortical neural progenitors (Cell Stem Cell) 1:12:00
• Image credit
• Letters read on TWiV 379 1:15:10

This episode is sponsored by 32nd Clinical Virology Symposium and Microbe Magazine Podcast 4:15, 1:05:25

Alan - Okeanos ExplorerDickson - Rome RebornVincent - Twitter Missing ManualScott - Best Science Images of 2015Kathy - Space Travel PostersRich - GoISSWatch

Peter - Antibiotics and the Problem of the Broken Market

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler

Guests: Greg Smith and Mark Fuccio

Greg Smith joins the TWiVirate to reveal how his lab discovered a switch that controls herpesvirus neuroinvasion, and then we visit the week's news about Zika virus.

• Dynamic ubiquitination drives herpesvirus neuroinvasion (PNAS)
• Releasing Zika virus data in real time (Nature)
• Zika virus infection and stillbirths (PLoS NTD)
• Zika virus infection, imported, Italy (Eurosurveillance)
• Letters read on TWiV 378

This episode is sponsored by 32nd Clinical Virology Symposium and Microbe Magazine Podcast

Alan - Science byline counting projectDickson - Bill Nye on climate changeVincent - Zdziarski's blog of things (Apple's motion to vacate)Greg - Atlas, the next generationKathy - Sources of Chaco woodMark - How Dogs Love Us by Gregory Berns

Bohdan - The real reason for brains

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler

The TWiVniks review the past week's findings on Zika virus and microcephaly, and reveal a chicken protein that provides insight on the restriction of transmission of avian influenza viruses to humans.

• Larvicide not involved in microcephaly (CBS News)
• Obama seeks $1.8b for Zika virus (Guardian)
• Zika virus in Puerto Rico (MMWR)
• Zika virus in semen (EID)
• Zika virus in Indonesia (EID)
• Phylogeny of Zika virus (EID)
• Zika virus may cause mental disease (NYTimes)
• Zika virus from amniotic fluid (Lancet)
• Host protein underlies avian influenza host restriction (Nature)
• Wendy Barclay on TWiV 177
• Home to roost (Cell)
• Image credit
• Letters read on TWiV 377

This episode is sponsored by 32nd Clinical Virology Symposium and Microbe Magazine Podcast

Alan - CDC vaccine quizDickson - A telescope so powerfulVincent - Virology Lectures 2016 (virology blog, iTunes U)Rich - Ion propulsion (Wiki)Kathy - Spurious Correlations

Johnye - Magellan telescope and van Egmond photograpy at Kids Should See This

Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler

Guest: Jeremy Luban

The TWiV team discusses the latest data on Zika virus, including ocular defects in infants with microcephaly, and isolation of the entire viral genome from fetal brain tissue.

• Ocular findings in infants with microcephaly (JAMA and Arq Bras Oft)
• Evidence of Zika virus infection in brain and placenta (MMWR)
• Zika virus associated with microcephaly (NEJM)
• Hard-core sequencing (Science)
• Zika virus and microcephaly (virology blog)
• Zika virus in the Americas (NEJM)
• JEV and WNV transplacental infections
• Rubella virus facts (WHO)
• Share your Zika virus reagents (virology blog)
• No link between anencephaly and Cache Valley Virus (EID)
• Image credit
• Letters read on TWiV 376

This episode is sponsored by 32nd Clinical Virology Symposium and ASM Microbe 2016

Alan - PhD Comics on gravitational wavesDickson - 25 years of HubbleVincent - Amazon AWS TOS and Confirming EinsteinRich - Mosquito mass productionKathy - TileAppJeremy - IL17A and autism and The Perfect Theory by Pedro Ferreira

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Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler

Guests: Carolyn Coyne, Helen Lazear, and Jeremy Luban

The TWiVziks present everything you want to know about Zika virus, including association of infection with microcephaly and Guillain-Barré syndrome, transmission, epidemiology, and much more.

• Zika outbreak in Brazil (EID) 13:40
• Zika summary (ProMedMail) 19:30
• Zika and microcephaly (MMWR) 27:30
• Facts about microcephaly (CDC)
• Brazil Ministry of Health microcephaly numbers
• Zika spread in Americas (MMWR)
• Deu Zica 42:30
• Microcephaly in Brazil (Lancet) 46:10
• GM mosquitoes did not start Zika outbreak (Discover) 1:31:30
• Debunking myths around Zika (Mad Virologist)
• Sexual transmission vs sexually transmissible (virology blog) 1:22:10
• CDC Zika travel guidance 1:15:45
• CDC on Zika and sex 1:17:10
• Image credit: Esper Kallas, University of Sao Paulo
• Letters read on TWiV 375

This episode is sponsored by 32nd Clinical Virology Symposium and ASM Grant Writing Webinar

Alan - Jetstream Online School for WeatherVincent - New virus watercolors from Michele Banks (one, two)Rich - The Mad Virologist (Facebook, blog)Kathy - Orcas in AntarcticaJeremy - Pardis Sabeti on TEDHelen - Science ValentinesCarolyn - Three letter word missing from Zika warnings

Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler

The TWiVniks consider the role of a cell enzyme that removes a protein linked to the 5'-end of the picornavirus genome, and the connection between malaria, Epstein-Barr virus, and endemic Burkitt's lymphoma.

• Divergent requirements for removing VPg (mBio)
• Bond, covalent bond (TWiV 210)
• Link between malaria and endemic Burkitt's lymphoma (PLoS Path)
• Multifactorial role of malaria in Burkitt's lymphoma (PLoS Path)
• Plasmodium infection promotes AID-dependent B cell lymphoma (Cell)
• Children's cancer dependent on climatic factors (Nature)
• Denis Burkitt (Wikipedia)
• Request for PACE trial data (virology blog)
• Letters read on TWiV 374

This episode is sponsored by 32nd Clinical Virology Symposium and ASM Grant Writing Webinar

Alan - Indoor skydivingVincent - Cancer Virus by Crawford, Johannessen, and RickinsonRich - WitKathy - The Only Woman in the Room by Eileen PollackDickson - Show everyone your clinical data

Send your virology questions and comments to twiv@microbe.tv

Host: Vincent Racaniello

Guest: Julius S. Youngner

Vincent speaks with Julius about his long career in virology, including his crucial work as part of the team at the University of Pittsburgh that developed the Salk inactivated poliovirus vaccine.

• Trypsin for cell cultures (Proc Soc Exp Biol Med)
• Interferon induction by nonviral stimuli (J Gen Physiol)
• Interferons of different molecular weight (Proc Soc Exp Biol Med)
• Poliovirus thermal mutants (J Bacteriol)
• Combination of different cells in culture (Science)
• Equine influenza vaccine (Eq Vet J)
• Younger role in Salk vaccine (interview, amednews)
• Cutter Incident (JRSM)
• Video of this episode - coming as soon as I can get home!

Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler

The TWiV-osphere introduces influenza D virus, virus-like particles encoded in the wasp genome which protect its eggs from caterpillar immunity, and a cytomegalovirus protein counters a host restriction protein that prevents establishment of latency.

• Ebolavirus update (ProMedMail)
• Influenza D virus (mBio)
• Vincent's influenza C virus paper (J Virol)
• Virus-like particles protect wasp eggs (Sci Adv)
• CMV protein foils cell defense against latency (Sci Adv)
• Image credit: Mark
• Letters read on TWiV 372

This episode is sponsored by ASM Grant Writing Webinar and ASM Biodefense

Alan - Go viralVincent - 3D virus popsicles (3ders, dangerous popsicles)Rich - Galileo thermometerKathy - Thing Explainer by Randall Munroe

Christiane - Using PowerPoint to shine

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler

The TWiVologists discuss the finding of a second transmissible cancer in Tasmanian devils, and development of new poliovirus strains for the production of inactivated vaccine in the post-eradication era.

• Second transmissible cancer in Tasmanian Devils (PNAS)
• New strains for production of IPV (PLoS Path)
• Synchronized switch to bivalent OPV (polioeradication.org)
• Virologists, start your poliovirus destruction (virology blog)
• Image is a painting by George Kush
• Letters read on TWiV 371

This episode is sponsored by ASM Grant Writing Webinar and ASM Biodefense

Alan - Skyfaring by Mark VanhoenackerVincent - Your Cells. Their Research. Your permission?Rich - NASA UV photos of the sunKathy - Aurora bike lightsDickson - Unfilled vials

Kevin - John Oliver on nutritional supplementsJim - Tour of Australian synchrotron

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Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler

The TWiVomics review ten captivating virology stories from 2015.

• Recoding to make vaccines and study viral functions: TWiV 351: The dengue codeTWiV 362: Gotta catch ’em all and new paper
• Defining the virome: TWiV 365: Blood, feuds, and a foodborne disease, TWiV 362: Gotta catch ’em all, TWiV 356: Got viruses?, TWiV 355: Baby’s first virome, TWiV 342: Public epitope #1, TWiV 323: A skid loader full of viromes
• Ebola outbreak wanes, vaccines tested, persistence noted: TWiV 361: Zombie viruses on the loose, TWiV 349: One ring to vaccinate them all, TWiV 341: Ebolavirus experiences, TWiV 336: Brought to you by the letters H, N, P, and Eye, TWiV 335: Ebola lite
• Spillovers and scares: TWiV 347: Rose rosette and squirrel roulette, TWiV 351: The dengue code, TWiV 364: It’s not SARS 2.0, TWiV 365: Blood, feuds, and a foodborne disease
• The “Gain of Function” debate continues: TWiV 321: aTrip and a pause, TWiV 351: The dengue code, TWiV 354: The cat in the HAART, TWiV 363: Eat flu and dyad, and the NSABB’s new draft report
• Detailed mechanisms of pathogenesis: TWiV 336: Brought to you by the letters H, N, P, and eye, and TWiV 345: How a vaccine got the nod - plus numerous episodes (324, 328, 329, 330, 339, 343, 349, 353, 369) where we talked about basic research that undergirds these clinically important discoveries.
• Dengue vaccines: Trial TWiV 319: Breaking breakbone. Dengvaxia was licensed in December in Mexico, Philippines, and Brazil (12/28); paper we didn’t have time to cover about broadly neutralizing Abs from viremic patients and another paper from same group with X-ray crystal structure of Ab + gpE.
• Why do some RNA viruses undergo antigenic variation and others do not? TWiV 340: No shift, measles
• TWiV 346: A double helical career
• TWiV 345: How a vaccine got the nod
• Letters read on TWiV 370

This episode is sponsored by ASM Microbe and ASM Biodefense

Alan - Scientifics onlineVincent - Giant squid in Tokyo HarborKathy - A Year of Sky

Daniel - Paper LifeStephen - How to deal with science denialistsVenkat - Kabisa

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Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler

A swarm of virologists discusses testing of a MERS coronavirus vaccine for camels, and how a neuronal stress pathway reactivates herpes simplex virus.

• MERS coronavirus vaccine for camels (Science)
• WHO MERS summary
• Neuronal stress pathway and herpes simplex virus reactivation (Cell Host Micr)
• Stress flips a chromatin switch (Cell Host Micr)
• How herpes simplex virus reactivates (UNC press release)
• Histone antibodies database
• New antibody portal (UNC press release)
• Image credit
• Letters read on TWiV 369

This episode is sponsored by Clinical Virology Symposium

Alan - SpaceX Falcon 9 landingVincent - Scientists: Engage the public! and Lego contest winnersKathy - Google Ngram viewer

Dennis - Americapox: The missing plagueMark - WeatherSpark and Quasispecies theory

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Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler

A plaque of virologists explores the biology of Zika virus and recent outbreaks, and the contribution of a filamentous bacteriophage to the development of biofilms.

Links for this episode

• More cowbell (Wikipedia)
• Microcephaly in Brazil (Outbreak News)
• Non-vector borne Zika transmission (EID)
• Zika virus outside Africa (EID)
• Zika virus possible sexual transmission (EID)
• Zika virus in saliva (J Clin Virol)
• Dengue's cousin Zika (Micr Inf)
• Filamentous phage promote biofilm assembly (Cell Host Micr)
• Biofilm history (MSU)
• Pseudomonas phage Pf1
• Image credit
• Letters read on TWiV 368

This episode is sponsored by Clinical Virology Symposium and ASM Microbe

Alan - Twelve days of norovirusVincent - This Week in EvolutionRich - In the Kingdom of Ice by Hampton SidesKathy - Kajanjian lecture on Frederick Novy and Milestones ceremony at UM

Peter - What if anti-vax, anti-science movement won?Stephen - Scandalous origin of vaccinesPritesh - Absurd way of doing business

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler

Guests: Carolyn Coyne and Coyne Drummond

Two Coynes join the TWiV overlords to explain their three-dimensional culture model of polarized intestinal cells for studying enterovirus infection.

• Carolyn and placentas on TWiV 193
• Three-dimensional cell culture model (mSphere)
• Bioreactor video (JoVE)
• Neurovirologist Richard T. Johnson, 84 (Hopkins and virology blog)
• Tardigrade HGT disputed (BioRxiv)
• Exaptation of archaeal virus capsid protein from Cas4 (Biol Dir)
• Image credit
• Letters read on TWiV 367

This episode is sponsored by ASM Microbe

Kathy - Tardigrade genome sequence (video)Alan - Antibiotic action nonprofit groupVincent - Ex Machina and genome editing moratoriumRich - Launch photography by Ben CooperKathy - HIV life cycle in video (paper)Dickson - 2015 Nobel Lectures Physiology or MedicineCarolyn - Metapneumovirus entry

Tom - Global host-pathogen databaseTrudy - Madame Curie by Eve Curie

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Host: Vincent Racaniello

Guests: Carla Giles, Zoe Dyson, Brianna McLean, and Caitlin O'Brien

In Melbourne, Australia, Vincent speaks with four PhD students about their research projects and what it's like to get a doctorate down under.

Video of this episode: view at YouTube

This episode is sponsored by ASM Microbe and ASM Biodefense.

Send your virology questions and comments to twiv@twiv.tv

TWiV is a MicrobeTV Production

Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler

For a TWiV Thanksgiving, Vincent, Alan, and Kathy trace the feud over genome editing, a new virus discovered in human blood, and the origins of hepatitis A virus.

• Battle over genome editing (Wired) 10:05
• First description of CRISPR in E. coli (J Bacteriol) 14:00
• Cas genes identified (Mol Micro) 14:20
• CRISPR-Cas provides immunity to bacterial viruses (Science) 15:35
• Cas9-crRNA complex mediates DNA cleavage (PNAS) 16:55
• Dual RNA-guided DNA endonuclease in bacterial immunity (Science) 17:50
• Genome engineering using CRISPR/Cas (Science) 18:50
• Cpf1 endonuclease (Cell) 23:10
• Classification of CRISPR/Cas systems (Nat Rev Micro) 23:50
• Summit on gene editing 21:25
• Novel human virus in transfusion recipients (mBio) 27:00
• Origins of hepatitis A virus in small mammals (PNAS) 55:05
• Image credit
• Letters read on TWiV 365 7:00, 1:20:35

This episode is sponsored by ASM Microbe, ASM Biodefense, and the 32nd Clinical Virology Symposium. (1:10, 1:18:30)

Kathy - Tardigrade genome sequence (video)Alan - XKCD on Gates polio eradication siteVincent - Lifting the Impenetrable Veil by Charles Calisher

Judi - Jennifer Doudna's Ted TalkRamon - Map of the Sky

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Hosts: Vincent Racaniello, Rich Condit, and Kathy Spindler

Guests: Ralph Baric and Vineet Menachery

Vincent, Rich, and Kathy speak with Ralph and Vineet about their research on the potential of SARS-like bat coronaviruses to infect human cells and cause disease in mice.

• Zoonotic potential of SARS-like bat viruses (Nature)
• SARS-CoV susceptibility loci via collaborative cross (PLoS Genetics) 47:00, 57:25
• Ebright and Wain-Hobson quotes (Nature) 1:17:20
• Misleading press release (UNC) 1:24:45
• Super powerful SARS 2.0 (not!) (Motherboard)
• SARS-like bat virus can jump without mutating (not!) (Daily Mail) 1:26:30
• Photo credit
• Letters read on TWiV 364 1:47:40

This episode is sponsored by Mt. Sinai Department of Microbiology (2:10), ASM Education (33:25), and the 32nd Clinical Virology Symposium (1:51:30)

Timestamps by Jennifer. Thanks!

Kathy - California Dept of Public HealthRich - Leafy greens on space stationVincent - Mechanical keyboards and TWiV on Perfect your podcast

Jesse - Networking for Nerds by Alaina G. LevineRussell - Worldmapper

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Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler

The TWiVers reveal influenza virus replication in the ferret mammary gland and spread to a nursing infant, and selection of transmissible influenza viruses in the soft palate.

• Photos of my visit to OHSU (Facebook) 6:30
• Folta departs (Facebook) 9:25
• T-VEC approved for melanoma (FDA) 14:45
• Talimogene laherparepvec (Wikipedia) 15:10
• T-VEC phase III trial (J Clin Oncol) 17:35
• How drugs are named (PopSci) 16:35
• Influenza transmission in mother-child dyad (PLoS Path) 23:10
• Soft palate and influenza transmission (Nature) 41:00
• Blausen 0872 UpperRespiratorySystem" by BruceBlaus. When using this image in external sources it can be cited as:Blausen.com staff. "Blausen gallery 2014". Wikiversity Journal of Medicine. DOI:10.15347/wjm/2014.010. ISSN 20018762. - Own work. Licensed under CC BY 3.0 via Commons." target="_blank">Image credit
• Letters read on TWiV 363 1:09:30

This episode is sponsored by Mt. Sinai Department of Microbiology, ASM Education, and ASM Biodefense and Emerging Diseases Conference

Timestamps by Jennifer. Thanks!

Kathy - Insect gearsAlan - Bye, Bye, EbolaRich - Hedy LamarrVincent - Should we resurrect extinct species?

Jennie - Bye, Bye, EbolaPeter - Epidemics on edX

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler

With their usual verve, the virus virtuosos illuminate a new method to identify all the viral nucleic acids in a sample, and regulation of viral gene expression by codon usage.

• Virome capture sequencing (mBio) 15:40
• Codon usage regulates viral gene expression (PNAS) 40:00
• Harold S. Ginsberg (Wikipedia) 7:15
• Letters read on TWiV 362 6:50, 1:03:20

This episode is sponsored by ASM Education: ASM MOOC 5:45

Timestamps by Jennifer. Thanks!

Kathy - Adam Savage, Andy Weir and Chris Hadfield talk about The MartianAlan - Project Apollo photo archive - and videoRich - Watch A Bowling Ball And Feather Falling In A VacuumDickson - California droughtVincent - Does AAAS care about Ebola anymore?

Anonymous - Lives saved by vaccinationPaul - Science articles: A guideJudi - How a virus invades your body

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Rich Condit

The TWiVsters discuss Frederick Novy's return from retirement to recover a lost rat virus, and evidence for persistence of Ebolavirus in semen.

• Frederick Novy and the rat virus (Ann Int Med) 18:20
• The rat virus (J Inf Dis) 22:55
• Recovery of the rat virus (J Inf Dis) 35:20
• Ebola sitrep 42:25
• Meningitis in Glaswegian Ebolavirus nurse (ProMedMail, Medscape) 45:50
• Sexual transmission of Ebolavirus (NEJM) 50:25
• Ebolavirus RNA persistence in semen (NEJM) 1:04:25
• Airplane banner falls into Hudson River (jpg)
• Image credit
• Letters read on TWiV 361 7:25, 1:13:25

This episode is sponsored by ASM Education: ASM MOOC 3:50

Alan - StickyRich - Arthur C. Clarke predicts the Internet (so did Vannevar Bush)Dickson - Nikon Small World Photomicrography winnersVincent - Pat Schloss on microbiome initiative

Awkward Skeptic - 16 year old wins Google prize for detecting Ebolavirus

Timestamps by Jennifer. Thanks!

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Hosts: Vincent Racaniello and Kathy Spindler

Guests: Michael Imperiale, Adam Lauring, and Akira Ohno

Vincent visits the University of Michigan where he and Kathy speak with Michael, Adam, and Akira about polyomaviruses, virus evolution, and virus assembly, on the occasion of naming the department of Microbiology & Immunology a Milestones in Microbiology site.

Links for this episode

• World Polio Day 3:20
• Mole Day 5:25
• The rat virus (J Inf Dis) 20:00
• Polyomavirus miRNAs (Curr Op Virol) 39:30
• Costs of GOF pause (mBio) 45:00
• Lethal mutagenesis of influenza virus (J Virol) 1:03:35
• Mutational robustness in RNA virus evolution (Nat Rev Micro) 52:00
• Membrane binding of gag (mBio) 1:12:55
• Cell proteins in retrovirus particles (J Virol) 1:23:15
• Michael's twitter
• Adam's twitter
• Video of this episode - view below or at YouTube

Kathy - Ada Lovelace and Charles Babbage Lego #1 and #2 and Ada Lovelace DayVincent - MiSciWriters

Junio - XKCD marks the spotKari - Spoonflower

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Host: Vincent Racaniello

Guest: Blossom Damania

Vincent speaks with Blossom about her laboratory's research on Kaposi's sarcoma-associated herpesvirus, including how it transforms cells, the switch between lytic and latent replication, and its interaction with the innate immune system of the host.

• Damania Laboratory
• Modulation of cGAS-STING by KSHV (PNAS)
• KSHV vIRF1 interacts with ISG15 pathway member (J Virol)
• KSHV NLR homolog inhibits inflammasome (Science)
• KSHV inhibition of PI3K and mTOR (Blood)
• KSHV confers survival advantage to endothelial cells (Cancer Res)
• UNC Global Oncology Program
• Blossom on Twitter
• Video of this episode - view at YouTube

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Host: Vincent Racaniello

Guest: Ileana Cristea

Vincent meets up with Ileana at Princeton University to talk about how her laboratory integrates molecular virology, mass spectrometry-based proteomics, and bioinformatics to unravel the interplay between virus and host.

• Cristea Laboratory
• Ileana's work previously on TWiV 269
• Blowing off steam (Cell Host Micr)
• Nuclear viral DNA sensors (J Biol Chem)
• mSystems
• Video of this episode - view at YouTube (coming soon!)

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler

Guest: Jens Kuhn

Jens joins the TWiVomics to discuss the strengths and weaknesses of viral taxonomy, including its history and evolution, how viruses are ordered, and why T. rex was classified without having a living isolate.

• Jens' previous TWiV (283) 5:15
• International Committee on the Taxonomy of Viruses (ICTV) 27:50
• ICTV 9th Report 32:55
• Classifying viruses by capsid architecture (Curr Opin Virol) 1:43:00
• Image credit
• Letters read on TWiV 357 10:55, 1:55:40

Timestamps by Jennifer. Thanks!

Alan - The Jefferson GridRich - Summer of ScienceKathy - Bill Nye dances The ClickDickson - The MartianJens - How I Killed Pluto, Life in Our Phage World, Why the Seahorse Tail is SquareVincent - Does the public trust science? (videos, summaries)

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler

Guest: Stephanie Neal

Stephanie joins the super professors to discuss the gut virome of children with serious malnutrition, caterpillar genes acquired from parasitic wasps, and the effect of adding chemokines to a simian immunodeficiency virus DNA vaccine.

• Invitrogen Science Hero Awards 15:05
• Gut DNA viromes of twins with severe malnutrition (PNAS) 34:30
• Random forests 48:00
• Wasps engineer caterpillars (PLoS Genetics) 50:20
• Chemokine-adjuvanted SIV DNA vaccine (Mucos Immunol) 1:02:20
• Guide to chemokines (pdf)
• Role for CCR10 in IgA secreting cell accumulation (J Immunol)
• CCL28 controls IgA plasma cell accumulation (J Exp Med)
• In vivo electroporation 1:07:25, 1:09:50
• Kathy's groan (jpg)
• Image credit
• Letters read on TWiV 356 14:55

Alan - Plates from Hooke's MicrographiaRich - The Wright Brothers by David McCulloughKathy - Spore evaporation-driven engines (video and article)Dickson - Color variations of Pluto and Lunar total eclipseStephanie - The science of moldy cheese by Carl ZimmerVincent - But Why?

Reed - The Bench Warmers Podcast

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler

The TWiV team considers the effect of a Leishmaniavirus on the efficacy of drug treatment, and the human fecal virome and microbiome in twins during early infancy.

• Announcing mSphere 5:50
• Leishmania virus influences drug outcome (paper 1, paper 2) 25:15
• Human gut virome and microbiome in infants (Nat Med) 46:00
• Multiple displacement amplification (Wiki) 46:00
• Virome of adult twins (Nature) 49:15
• Illumination by Kevin Folta 1:31:00
• Folta story at RetractionWatch 1:41:40
• Image credit
• Letters read on TWiV 355 9:40, 1:20:30

This episode is sponsored by ASMGAP 5:00

Timestamps by Jennifer. Thanks!

Alan - Vexed Muddler storeRich - Ivanpah Solar Power FacilityKathy - The real KermitDickson - Animal PortraitsVincent - Adventures of the Regatjes, Vaccines by Susan Nasif

Jacob - Great Wall of China keeps rabbits out

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler

The esteemed doctors of TWiV review a new giant virus recovered from the Siberian permafrost, why influenza virus gain of function experiments are valuable, and feline immunodeficiency virus.

• Non-viral proteins in vaccinia virus particles (Arch Virol) 8:15
• Thing a week (Jonathan Coulton, downloads) 23:20
• Immortal Life pornographic? Not! (HuffPost) 25:25
• Mollivirus sibericum (PNAS) 27:00
• Don't worry about Frankenvirus (WashPost) 27:10
• We need flu GOF research (article, Wisc State J, Science) 40:35
• Isolation of FIV (Science) 50:20
• FIV at Cornell Vet 1:06:25
• FIV latency (Retrovirol) 1:08:00
• FIV-receptor interactions (Curr Op Virol) 1:08:45
• Clin aspects FIV infection (Viruses)
• FIV antivirals (Viruses) 1:10:05
• How to treat FIV 1:10:45
• Bud's FIV therapy 1;10;50
• FIV vectors (CSH Prot) 1:13:50
• Lion FIV (Proc Biol Sci)
• FIV in cat species (J Virol) 1;01;45
• ACIP HVP vaccine recommendations (MMWR) 1:28:45
• HPV and cancer (CDC) 1:33:45
• Cervical cancer screening (pdf, CDC)
• Letters read on TWiV 354 7:45, 1:17:10

This episode is sponsored by ASMGAP

Timestamps by Jennifer. Thanks!

Alan - Experience CuriosityRich - Primrose SchoolKathy - Egg drop solutionsDickson - Dragonflies by Pieter van DokkumVincent - NASA guide to air-filtering houseplants

Amir - Publons (Nature article)

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler

The TWiVniacs discuss twenty-eight years of poliovirus shedding by an immunodeficient patient, and packaging of the innate cytoplasmic signaling molecule cyclic GMP-AMP in virus particles.

• 28 years of poliovirus shedding (PLoS Path) 36:50
• NOT a rare poliovirus (Wash Post) 44:35
• Ukraine polio outbreak (ProMedMail) 41:00
• Virus particle transfer of cGAMP (Science one, two) 47:50
• cGAS on TWiV 222 47:05
• Image credit
• Letters read on TWiV 353 6:30, 1:10:25

This episode is sponsored by ASMGAP 3:10

Timestamps by Jennifer. Thanks!

Alan - Miniature calendarKathy - Dr. Brainlove (prologue, v. 2.0, IndieGoGo)Dickson - The Ph.D. MovieVincent - Ghostery

Ramon - Children dieChris - ASMLive at ICAAC/ICC

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Host: Vincent Racaniello

Guest: Michele Banks

Vincent meets up with Michele Banks in Washington, DC to discuss her career as a creator of science-themed art.

• Michele Banks on Twitter
• Artologica
• Michele's blog
• The Finch and the Pea
• Joseph Cornell
• Not Exactly Rocket Science (Ed Yong)
• Tree of Life (Jonathan Eisen)
• Home Microbiome Study
• Kitten Microbiome Project
• Science Online
• The Vexed Muddler
• Luke Jerram
• A Daily Dish (Klari Reis)
• Neuroscience art (Greg Dunn)
• Ai Weiwei
• Video of this episode - coming soon
• Video of Michele working in her studio - view at YouTube

Send your virology questions and comments to twiv@twiv.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler

The Masters of the ScienTWIVic Universe discuss a novel poxvirus isolate from an immunosuppressed patient, H1N1 and the gain-of-function debate, and attenuation of dengue virus by recoding the genome.

• Paula Pitha-Rowe (Balt Sun)
• Novel poxvirus (CID)
• H1N1 and gain-of-function debate (mBio)
• Recoding dengue virus genome (PNAS)
• Attenuation by dinucleotide frequency change (PNAS)
• Codon pair and dinucleotide bias not distinguished (PNAS)
• Codon pair deoptimization artefact of CG/UA change (eLife)
• Can engineering subdue viral vaccine reversion? (Virus Evol)
• Attenuation by a thousand cuts (NEJM)
• Image credit
• Letters read on TWiV 351

Alan - Genetic roulette Rich - The Huntington Kathy - Simulated vomiting machine (video) Dickson - Last Cassini Saturn flybyVincent - Decaying Still Life (YouTube)

Ken - Cave meterology (Times, Nature)

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Host: Vincent Racaniello

Guest: Katherine A. High

Vincent speaks with Katherine High about her career and her work on using viral gene therapy to treat inherited disorders.

This episode is drawn from one of twenty-six video interviews with leading scientists who have made significant contributions to the field of virology, part of the new edition of the textbook Principles of Virology.

• Katherine A. High, MD
• AAV gene therapy for congenital blindness (Sci Trans Med)
• Gene therapy for hemophilia (Hematol)
• AAV capsid decoys (Sci Trans Med)
• Video of this episode - view at YouTube

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv

Hosts: Vincent Racaniello, Alan Dove, and Rich Condit

Vincent, Alan and Rich explain how to make a functional ribosome with tethered subunits, and review the results of a phase III VSV-vectored Ebolavirus vaccine trial in Guinea.

• Social media specialist position at ASM
• WHO dismisses Catholic church on vaccine safety
• U of T Dean resigns over anti-vaccine course (Tor Star)
• Vaccine Adverse Event Reporting System (VAERS)
• Countering anti-vaccination attitudes (PNAS)
• Designer ribosome (Nature)
• rVSV-EBOV preliminary report (NEJM)
• rVSV-EBOV phase III interim report (pdf, Lancet)
• World on verge of Ebolavirus vaccine (WHO)
• Ebolavirus vaccine in Africa (virology blog)
• Letters read on TWiV 349 11:55

This episode is sponsored by ASM GAP

Alan - Above and BeyondRich - DSCOVR (Dark moon side crossing Earth)Vincent - Stuxnet virus (Wired, YouTube)

Konrad - Stated Clearly

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Hosts: Vincent Racaniello and Rich Condit

Vincent and Rich discuss fruit fly viruses, one year without polio in Nigeria, and a permissive Marek's disease viral vaccine that allows transmission of virulent viruses.

• Virology tenure-track position at NCI (pdf)
• ASM Agar Art Contest
• Nigeria on brink of polio eradication (Nature)
• Dengue in Africa (Tyler Sharp)
• Permissive vaccines and virulence (PLoS Biol)
• Marek's disease vaccines and virulence (Ed Yong)
• Image credit
• Letters read on TWiV 348

This episode is sponsored by ASM GAP

Rich - Swan-Ganz catheter (Wiki, video)Vincent - Mobile DNA III

Kevin - Synthetic prions

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv

Hosts: Vincent Racaniello, Alan Dove, and Rich Condit

Vincent, Alan, and Rich discuss the virus behind rose rosette disease, and fatal human encephalitis caused by a variegated squirrel bornavirus.

• Virology tenure-track position at NIH (pdf) 7:00
• Voinnet investigation is a Swiss Federal secret (twitter) 12:25
• Rose rosette disease in Ft. Worth (Star Telegram) 29:35
• Rose rosette virus discovered (J Gen Virol) 34:25
• Emeravirus (ViralZone) 33:55
• Eriophyid mites (MI Botanical Garden) 32:40
• Mite vector of rose rosette disease (U Florida) 34:55
• Image credit
• Squirrel bornavirus associated with human encephalitis (NEJM) 38:40
• Poll: How do you consume podcasts? 1:21:40
• Letters read on TWiV 347 8:10, 59:40

This episode is sponsored by ASM GAP and ICAAC/ICC

Time stamps by Jennifer. Thanks!

Alan - National Immunization Awareness Month (Twitter campaign)Rich - Rosie Revere, Engineer by Andrea BeatyVincent - iPhone owns podcasts (9to5Mac)

Sandra - World Zoonoses Day graphicPeter - Mammal-carnivorous plant mutualism (bat and shrew)

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Hosts: Vincent Racaniello, Rich Condit, and Kathy Spindler

Guest: Joan Steitz

This episode was recorded at the 34th Annual Meeting of the American Society for Virology, where Vincent, Rich, and Kathy spoke with Joan Steitz, a tireless promoter of women in science and one of the greatest scientists of our generation.

• ASV annual meeting, Western University
• Steitz Laboratory at Yale University
• Steitz Laboratory on Twitter
• Panoramic photo of ASV TWiV by Matt Evans
• Photos of ASV2015 (Facebook)
• Video of this episode - view at YouTube

Kathy - Charity HallRich - Ribosome binding sites of phage R17 mRNAVincent - Pluto flyby

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler

The TWiVonauts review how the weather affects West Nile virus disease in the US, benefit of B cell depletion for ME/CFS patients, and an autoimmune reaction induced by influenza virus vaccine that leads to narcolepsy.

• Virology faculty position, University of Toledo
• Weather and West Nile virus disease (AJTMH)
• B cell depletion benefits ME/CFS patients (PLoS One)
• Antibodies to influenza nucleoprotein cause narcolepsy (Science Transl Med)
• Letters read on TWiV 345

This episode is sponsored by ASM GAP - turn your science into a company

Dickson - Algal DMS releasing enzymeAlan - Science of beer agingKathy - The Art of Science winnersVincent - The future of Lego plastics

Eric - Economics in One Lesson by Henry HazzilitStephen - The Martian by Andy Weir (audiobook)

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Host: Vincent Racaniello

Guests: Ruth Jarrett, Glen Nemerow, and Esther Schnettler

At the Glasgow Science Festival microTALKS, Vincent speaks with Ruth, Glen, and Esther about their research on viruses and Hodgkin lymphoma, adenovirus structure and entry into cells, and interactions between arthropod borne viruses and their hosts.

• Glasgow Science Festival microTALKS
• Hodgkin lymphoma (Histopath)
• HHV-6 and Hodgkin lymphoma (PLoS One)
• Adenovirus entry (PLoS Path)
• Adenovirus membrane penetration (Virology)
• Mosquito pathways that limit CHIK replication (PLoS Negl Trop Dis)
• Tick antiviral RNAi (Nucl Acids Res)
• Video of this episode - view at YouTube

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler

The TWiVerinoes discuss the potential for prion spread by plants, global circulation patterns of influenza virus, and the roles of Argonautes and a viral protein in RNA silencing in plants.

• Prions in plants (Cell Rep) 11:35
• Global influenza virus circulation (Nature) 20:40
• Rough patch for plant RNA silencing (Lab Times) 29:50
• Role of Argonaute proteins in plant viral defense (PLoS Path) 32:15
• Rub-inoculation (YouTube) 42:45
• Image credit
• Letters read on TWiV 343 4:00, 1:11:45

Timestamps by Jennifer. Thank you!

Dickson - Global rainfall and snowfall map and A Week in the Life of RainAlan - Automated patch clampKathy - More Women in Science mini LegosRich - The Martian trailerVincent - It's a bug's life

May - p53 by Sue Armstrong

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Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler

The TWiVniks discuss the structure of a virus that reproduces in an extreme environment, long-term consequences of Ebolavirus infection, and VirScan, a method to identify the different virus infections you have had in your lifetime.

• Virus with A-form DNA (Science)
• Viruses in the extreme (virology blog)
• Sequelae of Ebolavirus infection (Lancet Inf Dis)
• Long term Ebolavirus effects (virology blog)
• Take the chili ME challenge
• Serological profiling of human viral infections (Science)
• Your viral past (virology blog)
• Letters read on TWiV 342

Timestamps by Jennifer. Thank you!

Dickson - Incredible photosAlan - Alone in a room full of science writersKathy - Calico cats and Siamese catsRich - Missing link in evolutionVincent - Podcasts saving NPR and Podcasting blossoms

Jenny - What Bill Gates is afraid ofNeva - The Kardashian Index

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